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| Status |
Public on Feb 18, 2018 |
| Title |
Plant-specific histone residue F41 restricts H3.1 distribution in heterochromatin |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The plant specific H3.1F41 plays crucial role in H3.1 genome-wide deposition pattern.
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| Overall design |
We made single and double mutant transgenic lines between histone 3 variants H3.3 and canonical H3.1 in Arabidopsis. The mutated constracts were fused with FLAG tag and transformed into Arabidopsis driven by their endougenous promoter. ChIP seq experiments were did by anti FLAG tag (Sigma (M8823)).
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| |
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| Contributor(s) |
Lu L, Zhong X |
| Citation(s) |
29434220 |
| |
| Submission date |
Jan 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Xuehua Zhong |
| Organization name |
Wisconsin Institute for Discovery
|
| Department |
Epigenetics
|
| Street address |
330 N. Orchard St.
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA360334 |
| SRA |
SRP096152 |
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