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| Status |
Public on May 19, 2017 |
| Title |
Distinct roles for matrix metalloproteinases 2 and 9 in hematopoietic stem cell emergence, migration and niche colonization |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report gene expression data for FACS sorted zebrafish mpeg1:mCherry + and mpx:EGFP + cells collected from whole embryos at 72 hours post fertilization (hpf). We also report gene expression data for the remaining, transgene negative, portion of these embryos.
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| Overall design |
~1,000 mpeg1:mCherry+; mpx:EGFP+ transgenic embryos were homogenized, filtered, and sorted using FACS into PBS, collecting >50,000 cells for each of the three populations: mpeg1:mCherry+, mpx:EGFP+ and double negative (no double positive cells were collected as there was almost no overlap between mCherry and EGFP expression).
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| Contributor(s) |
Hagedorn EJ, Yang S, Zon L |
| Citation(s) |
28416284 |
| |
| Submission date |
Jan 18, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Leonard Zon |
| E-mail(s) |
zon@enders.tch.harvard.edu
|
| Organization name |
Boston Children's Hospital
|
| Department |
Oncology/Hematology
|
| Street address |
1 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
|
| Samples (3) |
| GSM2462945 |
zebrafish mpeg1:mCherry +, 72 hpf [EH_R] |
| GSM2462946 |
zebrafish mpx:EGFP +, 72 hpf [EH_G] |
| GSM2462947 |
zebrafish double negative, 72 hpf [EH_N] |
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| Relations |
| BioProject |
PRJNA362479 |
| SRA |
SRP097006 |
