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| Status |
Public on Jan 31, 2008 |
| Title |
Late-term turkey embryo liver gene expression profiling |
| Platform organism |
Gallus gallus |
| Sample organism |
Meleagris gallopavo |
| Experiment type |
Expression profiling by array
|
| Summary |
Turkey embryos are very sensitive to perturbations in energy metabolism because they have a wider hatching window than chicken embryos. Mortality of turkey embryos during late-term incubation is high relative to chickens, and many surviving hatchlings have compromised vitality. Intestinal maturation at hatch is also crucial to survival and post-hatch performance. The study of poultry embryo metabolism during the last stages of incubation is difficult due to many shifts and changes that occur in preparation for hatching. Microarray technology is suitable to study complex biological systems like avian late-term embryonic development. Therefore, the objectives of this study were to create a customized focused oligonucleotide microarray based on chicken genome sequences that could be used to study late-term avian metabolism and intestinal maturation, and use this array to survey turkey embryos gene expression from 20 days of incubation until hatch. The key features of this microarray are that all genes present have been annotated and gene spot replication (4) within each array chip. Microarray analysis was performed on liver, pectoral muscle, hatching muscle, and duodenum
Keywords: time course, embryo development
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| Overall design |
Pooled samples from 6 embryos were arraged on a complete interwoven loop design where each treatment (embryonic ages 20, 22, 24, 26 and 28) was replicated 4 times (2 dye-swaps).
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| |
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| Contributor(s) |
de Oliveira JE, Ashwell CM, Ferket PR, Uni Z |
| Citation(s) |
23472025 |
| |
| Submission date |
Oct 22, 2007 |
| Last update date |
Jun 14, 2013 |
| Contact name |
Jean Eduardo de Oliveira |
| Phone |
919-515-5537
|
| Fax |
919-515-7070
|
| URL |
http://www.ncsu.edu/ashwell-lab/
|
| Organization name |
North Carolina State University
|
| Department |
Poultry Science
|
| Lab |
Nutrition/Domestic Animal Genomics
|
| Street address |
2711 Founders Dr
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695-7608 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6041 |
NCSU_chicken_JS1 (96 oligos) |
|
| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE9475 |
Late-term turkey embryo liver, pectoralis muscle, hatching muscle and duodenum gene expression |
|
| Relations |
| BioProject |
PRJNA105149 |
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