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Series GSE94098 Query DataSets for GSE94098
Status Public on Apr 20, 2017
Title FTO regulates adult neurogenesis through modulating the dynamics of N6-methyladenosine
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Fat mass and obesity-associated gene (FTO) is a member of the Fe (II) and oxoglutarate-dependent AlkB oxygenase family, and has been linked to obesity and intellectual disability. However, the role of FTO in neurodevelopment and neurogenesis remains largely unknown. Here, we show that FTO is expressed in adult neural stem cells (aNSCs) and neurons, and displays dynamic expression during postnatal neurodevelopment. The loss of FTO leads to the decreased brain size and body weight. We found that FTO deficiency could reduce the proliferation and neuronal differentiation of aNSCs in vivo, which leads to the impaired learning and memory. Given the role of FTO as a demethylase of N6-methyladenosine (m6A), we further performed the genome-wide m6A profiling and observed the dynamic m6A modification during postnatal neurodevelopment. The loss of FTO could lead to the altered m6A modifications on the mRNAs of several key components of BDNF pathway. These results together suggest that FTO play important roles in neurogenesis and learning and memory.
Overall design Gene expression analyses were performed on mRNA samples isolated from the hippocampi of adult Fto knockout mice (8-12 weeks old) and age/gender-matched wild-type C57BL/6 mice. N6-methyladenosine (m6A) epitranscriptome profiling was performed on mRNA samples isolated from the hippocampi of 2-week-old and 6-week-old male wild-type C57BL/6 mice.
Web link
Contributor(s) Zhang F, Jin P
Citation(s) 28398475
Submission date Jan 26, 2017
Last update date May 15, 2019
Contact name Feiran Zhang
Organization name Emory University
Department Human Genetics
Lab Peng Jin
Street address 615 Michael St NE
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM2468909 RNA-seq, 2-week-old WT mice, hippocampus, input, replicate 1
GSM2468910 RNA-seq, 2-week-old WT mice, hippocampus, input, replicate 2
GSM2468911 RNA-seq, 2-week-old WT mice, hippocampus, m6A-IP, replicate 1
BioProject PRJNA368849
SRA SRP097869

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94098_RAW.tar 379.2 Mb (http)(custom) TAR (of FPKM_TRACKING, TDF, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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