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| Status |
Public on Apr 01, 2017 |
| Title |
An in vitro model of intestinal infection reveals a developmentally regulated transcriptome of Toxoplasma sporozoites and a NF-κB-like signature in infected host cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Toxoplasmosis is a zoonotic infection affecting approximately 30% of the world’s human population. After sexual reproduction in the definitive feline host, Toxoplasma oocysts, each containing 8 sporozoites, are shed into the environment where they can go on to infect humans and other warm-blooded intermediate hosts. Here, we use an in vitro model to assess host transcriptomic changes that occur in the earliest stages of such infections. We show that infection of rat intestinal epithelial cells with mature sporozoites primarily results in higher expression of genes associated with Tumor Necrosis Factor alpha (TNFα) signaling via NF-κB. Furthermore, we find that, consistent with their biology, these mature, invaded sporozoites display a transcriptome intermediate between the previously reported day 10 oocysts and that of their tachyzoite counterparts. Thus, this study uncovers novel host and pathogen factors that may be critical for the establishment of a successful intracellular niche following sporozoite-initiated infection.
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| Overall design |
Rat intestinal epithelial cells were infected in duplicate with either Toxoplasma sporozoites or tachyzoites in the presence or absence of frozen sporozoites for 8 hours. Frozen sporozoites and lysates from uninfected cells were used as controls. The experiment was performed twice. Total RNA was extracted from the resulting 24 samples and the transcriptional profiles of both host and parasites were determined using RNA sequencing on the Illumina NextSeq platform
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| Contributor(s) |
Sagawa J, Fritz HM, Boothroyd JC |
| Citation(s) |
28362800 |
| Submission date |
Feb 03, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Pascale S Guiton |
| E-mail(s) |
pguiton@stanford.edu
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| Organization name |
Stanford University
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| Department |
Microbiology and Immunology
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| Lab |
Dr. John Boothroyd
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| Street address |
Sherman Fairchild Science Building Stanford School of Medicine 299 campus Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5124 |
| Country |
USA |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA369798 |
| SRA |
SRP098807 |
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