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| Status |
Public on Apr 25, 2018 |
| Title |
A B-ARR-mediated cytokinin transcriptional network directs hormone-cross regulation and shoot development |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
While the biosynthesis, degradation, and signaling pathways for the essential plant hormone cytokinin have been extensively studied, the direct transcriptional targets of B-type ARRs, the key TFs essential for cytokinin action have not been defined. We epitope tagged four B-ARRs (1, 10, 12, and 14) using recombineering techniques and profiled TF binding sites using chromatin immunoprecipitation sequencing (ChIP-seq). The resulting cytokinin transcriptional response network allowed construction of the first cytokinin TF network, identification of numerous direct downstream targets along with the B-ARR-6BA motif and elucidation of one mechanism of activation of WUSCHEL, which requires a high concentration of cytokinin in shoot apical meristem for TF binding.
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| Overall design |
we present the profiles of in vivo DNA binding sites for the genetically redundant type-B ARABIDOPSIS RESONSE REGULATORS (B-ARRs): ARR1, ARR10, and ARR12 along with ARR14 using recombineering YFP tagged lines and subsequent chromatin immunoprecipitation sequencing (ChIP-seq) analysis. The expression and genomic protein localization of B-ARRs overlap extensively, demonstrating numerous common targets among the B-ARRs. The negative regulators of cytokinin signaling, the type-A ARRs (A-ARRs), are the top ranked targets of B-ARRs. These findings confirm an immediate negative B-ARR-driven feedback loop in cytokinin signaling. Constructing a cytokinin primary response transcriptional network revealed a recurring theme involving extensive cross-regulation both within the components of the cytokinin pathway and with other plant hormone response pathways.
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| Contributor(s) |
Xie M, Chen H, O'Neil R, Huang C, Ecker JR |
| Citation(s) |
29686312 |
| |
| Submission date |
Feb 03, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Joseph R Ecker |
| E-mail(s) |
ecker@salk.edu
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| Phone |
8584534100
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| Organization name |
HHMI-Salk-Institute
|
| Department |
Genomic Analysis Laboratory
|
| Lab |
Ecker lab
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| Street address |
10010 North Torrey Pines Road
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA369824 |
| SRA |
SRP098817 |
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