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| Status |
Public on Sep 25, 2019 |
| Title |
Oncogenic zinc finger protein ZNF322A promotes lung cancer stemness through transcriptionally suppressing c-Myc expression [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ZNF322A, a C2H2 zinc finger transcription factor, is an oncoprotein in lung cancer. However, the transcription mechanisms of ZNF322A in lung cancer stemness remain elusive. By integrating our chromatin immunoprecipitation-sequencing and RNA-sequencing datasets, we identified and validated transcriptional targets of ZNF322A, which significantly enriched in developmental processes. Indeed, overexpression of ZNF322A promoted self-renewal ability and increased stemness-related gene expressions in vitro and in vivo. Importantly, ZNF322A bound directly to c-Myc promoter to transcriptionally suppress c-Myc expression, which in turn increased mitochondrial oxidative phosphorylation, promoted cell motility and thus maintained lung cancer stemness properties. Clinically, ZNF322AHigh/c-MycLow expression profile was revealed as an independent factor for poor outcome of lung cancer patients. Our study provides first evidence that ZNF322A-centered transcriptome promotes lung tumorigenesis and ZNF322A acts as a transcription suppressor of c-Myc to maintain lung cancer stemness by shifting metabolism phenotype to oxidative phosphorylation.
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| Overall design |
Genome-wide ZNF322A binding sites were identified via chromatin immunoprecipitation-sequencing analysis of vecoter control and HA-tagged ZNF322A overexpressing H460 lung cancer cells. ChIP-seq analyses were performed in duplicated samples using Applied Biosystems SOLiD system.
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| |
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| Contributor(s) |
Wang Y, Jen J |
| Citation(s) |
30258097 |
| |
| Submission date |
Feb 08, 2017 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Yi-Ching Wang |
| E-mail(s) |
ycw5798@mail.ncku.edu.tw
|
| Phone |
+886-6-2353535
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| Organization name |
National Cheng Kung University
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| Department |
Department of Pharmacology, College of Medicine
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| Street address |
No.1, University Road, Tainan 70101, Taiwan, R. O. C.
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| City |
Tainan |
| ZIP/Postal code |
70101 |
| Country |
Taiwan |
| |
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| Platforms (1) |
| GPL16288 |
AB 5500xl Genetic Analyzer (Homo sapiens) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE94657 |
Oncogenic zinc finger protein ZNF322A promotes lung cancer stemness through transcriptionally suppressing c-Myc expression |
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| Relations |
| BioProject |
PRJNA373804 |
| SRA |
SRP099131 |
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