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| Status |
Public on Sep 05, 2017 |
| Title |
Endogenous Androgen Receptor proteomic profiling reveals genomic subcomplex involved in prostate tumorigenesis |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Androgen Receptor (AR) is a key player in prostate cancer development and progression. Here, we applied immunoprecipitation mass spectrometry of endogenous AR in LNCaP cells to identify individual components of the AR transcription complex. In total, 66 known and novel AR interactors were identified in the presence of R1881, which were critically and selectively required in AR-driven prostate cancer cell proliferation. AR interactors required for LNCaP proliferation were profiled using ChIP-seq, identifying distinct genomic subcomplexes of AR interaction partners. Interestingly, three major subgroups of genomic subcomplexes were identified, where a selective gain-of-function for AR was identified in tumorigenesis, dictated by FOXA1 and HOXB13. Jointly, our study reveals subclasses of the AR transcription complex formation and composition, that is dictated by overexpression of AR-interactors, resulting in reprogramming of the AR cistrome and interactome in a genomic location-specific manner.
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| Overall design |
AR, ARID1a, BRG1, FOXA1, HOXB13, TLE3, TRIM28 and WDHD1 binding to DNA was profiled by ChIP-seq (Chromatin Immunoprecipitation) genome-wide in prostate cancer cell line LNCaP.
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| Contributor(s) |
Stelloo S, Zwart W |
| Citation(s) |
28925401, 31855178 |
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| Submission date |
Feb 08, 2017 |
| Last update date |
Jan 27, 2020 |
| Contact name |
Suzan Stelloo |
| E-mail(s) |
Stelloo@science.ru.nl
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| Organization name |
Radboud University
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| Department |
Molecular Biology
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| Street address |
Geert Grooteplein Zuid 28
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| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA371800 |
| SRA |
SRP099063 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE94682_RAW.tar |
9.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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