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| Status |
Public on Jun 21, 2018 |
| Title |
Comparison of miRNA expression profile among the livers of C57BL/6J control mice, high fat diet (HFD) mice and metformin treated mice |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Nonalcoholic fatty liver disease (NAFLD) is a growing worldwide epidemic and an important risk factor for the development of insulin resistance, type 2 diabetes, nonalcoholic steatohepatitis (NASH), and hepatic cellular carcinoma (HCC). Despite the prevalence of NAFLD, lifestyle interventions involving exercise and weight loss are the only accepted treatments for this disease. Over the past years, metformin has been shown to improve NAFLD in preclinical animal models. Hepatic microRNAs (miRNAs) have been suggested as therapeutic targets because of their involvement in the progression of NAFLD. The aim of this study was to identify miRNAs associated with the regulation of liver lipid metabolism after metformin treatment. We performed computational analysis of the function enrichment and disease association of the differentially expressed miRNAs and their targets. Our analysis may shed light on the novel treatment method for NAFLD.
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| Overall design |
Three-week-old wild-type C57BL/6J mice were purchased from the Spfanimals Company (Beijing, China). These mice were divided into three groups: the control group (C), the high fat diet group (H) and the metformin treated (M) group. For the normal control (C) group, the mice were maintained with chow diet for 17 weeks. For the high fat diet (H) and metformin treated (M) group, C57BL/6J mice were fed a high fat diet (HFD, D12451, 45% kcal from fat, Research Diet, USA, http://www.researchdiets.com/opensource-diets/stock-diets) for 17 weeks. Starting at the 15 weeks old of age (i.e. after 12 weeks of high fat diet feeding), the high fat diet (H) and metformin treated (M) group were differentially treated: the mice from metformin treated (M) group were intragastrically treated with 3 mg/kg/day metformin for 5 weeks; while those from the high fat diet (H) group were treated with saline vehicle for 5 weeks. Finally, the liver tissue from different groups of mice were collected respectively at the 20 weeks old of age, and the liver miRNA expression profiles were measured using the Agilent mouse miRNA 21.0 microarray. Three biological replicates were prepared for each group. It is noteworthy that each biological replicate contains a mixed liver tissue samples collected from four mice of the same group.
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| Contributor(s) |
Guo J, Zhou Y, Cheng Y, Fang W, Sun M, Cui Q, Li J |
| Citation(s) |
29466788 |
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| Submission date |
Feb 10, 2017 |
| Last update date |
Jun 23, 2018 |
| Contact name |
Yuan Zhou |
| E-mail(s) |
zhouyuanbioinfo@hsc.pku.edu.cn
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| Organization name |
Peking University
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| Department |
Department of Biomedical Informatics and Department of Physiology and Pathophysiology, School of Basic Medical Sciences
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| Street address |
38 Xueyuan Rd, Haidian District
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| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (1) |
| GPL21265 |
Agilent-070155 Mouse miRNA Microarray (miRBase Release 21.0, miRNA ID version) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE94844 |
Microarray profiling of non-coding RNAs and mRNA expression among the livers of C57BL/6J control mice, high fat diet (HFD) mice and metformin treated mice |
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| Relations |
| BioProject |
PRJNA374349 |
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