|
| Status |
Public on Apr 11, 2017 |
| Title |
Sequencing the DNA and mRNA from the same single cell |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Single-cell genome and transcriptome data can address many questions, including somatic variation, meiotic recombination and cell-to-cell heterogeneity of expression, and techniques for single-cell sequencing have developed rapidly in recent years. Here, we further adapted this technology by performing simultaneous single-cell exome and transcriptome profiling on individual mouse oocytes. In brief, we physically separated the oocyte nuclei and cytoplasm using a micromanipulator. We then conducted exome-sequencing on the isolated nuclei and mRNA-sequencing on the enucleated cytoplasm. Combined analysis of the simultaneously derived sequencing data disclosed a high heterozygous recombination rate during oocyte meiosis, and genomic and transcriptomic heterogeneity among individual oocytes. This approach provides unprecedented opportunities to study RNA editing of single oocytes.
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| |
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| Overall design |
Sequencing the DNA and mRNA from 6 single mouse secondary oocytes
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| |
|
| Contributor(s) |
Wang L, Guo J, Cao W, Zhang M, He J, Li Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Zhoufang Li |
| E-mail(s) |
lizf@sustc.edu.cn
|
| Organization name |
SUSTech
|
| Department |
Department of Biology
|
| Lab |
Jiankui He Lab
|
| Street address |
No 1088,xueyuan Rd., Xili, Nanshan District,Shenzhen,Guangdong,China
|
| City |
ShenZhen |
| ZIP/Postal code |
518055 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA374500 |
| SRA |
SRP099407 |
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