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| Status |
Public on Jan 01, 2018 |
| Title |
Expression data from primary keratinocytes obtained from WT and keratinocyte specific Glut1-deficient mice [MTA-1_0] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Glut1 is highly expressed in basal cells of keratinocytes, but the regulation of Glut1 and expression of additional glucose transporters in the skin has not been explored, here we specifically ablate Glut1 in epidermal keratinocytes to elucidate the role of glucose transport in the keratinocytes.
we performed microarray analysis in WT and Glut1 deficient primary keratinocytes to determine the pathways might contribute to the impaired proliferation in Glut1 deficient keratinocytes.
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| Overall design |
We harvest primary keratinocytes from 1 week old WT (n=4) and keratinocyte specific Glut1-deficient (n=4) mice, keratinocytes from each mice were seeded at one 10 cm dishes in complete KSFM medium. After 3 days, digest the cells, reseed the cell in new dishes with same number. The cells were harvested the next day. Two plates of cells of the same genotype were randomly harvested together, thus each genotype have 2 samples for microarray analysis.
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| Contributor(s) |
Zhang Z, Wang R |
| Citation(s) |
29662201 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR072655 |
Mechanisms of Glucose Dependence in Proliferating Cells |
UT SOUTHWESTERN MEDICAL CENTER |
Richard C Wang |
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| |
| Submission date |
Feb 14, 2017 |
| Last update date |
Apr 18, 2018 |
| Contact name |
Richard C Wang |
| Organization name |
UT Southwestern
|
| Department |
Dermatology
|
| Lab |
Wang Lab
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20775 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CSV version] |
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| Samples (4)
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| GSM2490516 |
Keratinocytes WT, biological rep1_MTA-1_0 |
| GSM2490517 |
Keratinocytes WT, biological rep2_MTA-1_0 |
| GSM2490518 |
Keratinocytes KO, biological rep1_MTA-1_0 |
| GSM2490519 |
Keratinocytes KO, biological rep2_MTA-1_0 |
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| This SubSeries is part of SuperSeries: |
| GSE102955 |
Expression data from primary keratinocytes obtained from WT and keratinocyte specific Glut1-deficient mice |
|
| Relations |
| BioProject |
PRJNA374718 |
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