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Series GSE94990 Query DataSets for GSE94990
Status Public on Apr 26, 2017
Title A Reciprocal Regulatory Loop Between TAZ/YAP and G protein Gαs Regulates Schwann Cell Proliferation and Differentiation
Organisms Mus musculus; Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Schwann cell (SC) myelination in the peripheral nervous system is essential for motor function, and uncontrolled SC proliferation occurs in cancer. Here, we show that a dual direct role for Hippo effectors TAZ and YAP in regulation of SC proliferation and myelination through modulating G protein expression and interacting with SOX10, respectively. Developmentally-regulated mutagenesis indicates that TAZ/YAP are critical for SC proliferation yet also required for their differentiation and myelination. Genome-wide occupancy mapping and transcriptome profiling reveal that nuclear TAZ and YAP promote SC proliferation by activating cell cycle regulators, while targeting critical differentiation regulators in cooperation with SOX10 for myelination. We further identified that TAZ targets and represses Gnas, encoding Gαs-protein, which opposes TAZ/YAP activities to decelerate proliferation. Gnas deletion expands SC precursor pools and blocks myelination in the sciatic nerve. Thus, our study revealed that the Hippo/TAZ/YAP and Gαs-protein feedback circuit functions as a fulcrum balancing SC proliferation and differentiation, providing insights into molecular programming of SC lineage progression and homeostasis.
 
Overall design 3 Control Samples and 3 Mutant
 
Contributor(s) Deng Y, Wu LM, Bai S, Lu Q
Citation(s) 28443644
Submission date Feb 16, 2017
Last update date May 15, 2019
Contact name Richard Lu
Organization name Cincinnati Children's Hospital Medical Center
Department CBDI
Lab Lu Lab,T6.525
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (13)
GSM2493834 P5_Ctr-Gnas
GSM2493835 P5_Cko-Gnas
GSM2493836 P5_Ctr-TazYap-1
Relations
BioProject PRJNA375093
SRA SRP100075

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94990_RAW.tar 1.1 Gb (http)(custom) TAR (of FPKM_TRACKING, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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