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| Status |
Public on Feb 13, 2019 |
| Title |
Human Long Intergenic Noncoding RNA Linc-ADAL Knockdown in Mature Adipocytes using Lentiviral ShRNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Thousands of human long intergenic noncoding RNAs (lincRNAs) have been detected in human adipose tissue. Here we characterized the function of one human adipse lincRNA, linc-ADAL (chr5:115292235-115296985) in mature adipcoytes through loss-of-function studies. Our results indicate that knockdown of linc-ADAL in differentiated adipocytes modulated expression of lipid metabolism genes.
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| Overall design |
Lentiviral based shRNAs were used to knockdown linc-ADAL in differentiated ASC-derived adipocytes. ASC-adipoctes were transduced on day 8 of adipogenic differentiation. Cells were harvested for RNA extraction on day 6 post-transduction.
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| Contributor(s) |
Xuan Z, Chenyi X |
| Citation(s) |
29925637 |
| |
| Submission date |
Feb 22, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Chenyi Xue |
| Organization name |
Columbia University
|
| Street address |
630 W 168th St.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA376272 |
| SRA |
SRP100501 |
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