GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE95173 Query DataSets for GSE95173
Status Public on Feb 13, 2019
Title Human Long Intergenic Noncoding RNA Linc-ADAL Knockdown in Mature Adipocytes using Lentiviral ShRNA
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Thousands of human long intergenic noncoding RNAs (lincRNAs) have been detected in human adipose tissue. Here we characterized the function of one human adipse lincRNA, linc-ADAL (chr5:115292235-115296985) in mature adipcoytes through loss-of-function studies. Our results indicate that knockdown of linc-ADAL in differentiated adipocytes modulated expression of lipid metabolism genes.
Overall design Lentiviral based shRNAs were used to knockdown linc-ADAL in differentiated ASC-derived adipocytes. ASC-adipoctes were transduced on day 8 of adipogenic differentiation. Cells were harvested for RNA extraction on day 6 post-transduction.
Contributor(s) Xuan Z, Chenyi X
Citation(s) 29925637
Submission date Feb 22, 2017
Last update date May 15, 2019
Contact name Chenyi Xue
Organization name Columbia University
Street address 630 W 168th St.
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM2498225 adipocyte_control_rep1
GSM2498226 adipocyte_control_rep2
GSM2498227 adipocyte_control_rep3
BioProject PRJNA376272
SRA SRP100501

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE95173_RAW.tar 963.2 Mb (http)(custom) TAR (of BW)
GSE95173_adipocyte_ctrl_shR.diff.gz 910.7 Kb (ftp)(http) DIFF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap