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| Status |
Public on Oct 01, 2021 |
| Title |
Host Genes Differentially Expressed by Virulent and Attenuated Strains of Japanese Encephalitis Virus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus and causes acute encephalitis with high mortality rate in humans. Despite the demonstration of difference in neuroinvasiveness between JEV strains, little is known about host gene response upon infection of virulent and attenuated JEV strains. To understand the pathogenesis mechanism of JEV infection, we performed a comparative genome-wide microarray analysis (Affymetrix, mouse genome 430 2.0 with 45101 probe sets) of genes expressed in the mice brains three days post intracerebrally inoculated with JEV strains of different neurovirulence. We found that the virulent strain of JEV (RP-9) tended to affect the host gene expression more profoundly than the attenuated one (RP-2ms), as 961 vs. 665 and 655 vs. 509 genes were at least two-fold upregulated and downregulated by RP-9 and RP-2ms, respectively. In order to understand the host defense response against JEV, we further analyzed the microarray data with special focus on immune response. The expression levels of selected genes were further verified by quantitative RT-PCR at 1-, 3- and 5-day post JEV infection. The differentially regulated genes reported here likely contribute to the neurovirulent phenotype by modulating the host immunity, viral replication and/or cytotoxicity.
Keywords: day3, virus (1 x 10^4 PFU), intracerebrally (ic) inoculated, brain total RNA
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| Overall design |
Mice: 4~5-week-old C57BL/6.
Virus: JEV RP-9 (1 x 10^4 PFU), JEV RP2-ms (1 x 10^4 PFU), PBS (as control), intracerebrally (ic) inoculated.
Time course: The mice were sacrificed on day-1, day-3, and day-5 post-inoculation, and RNA from brain tissues were harvested by TRIZOL Reagent (Invitrogen).
DNA chip: Affymetrix Mouse Genome 430 2.0.
Data analysis: GeneSpring 7.2 (Silicon Genetics).
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| Contributor(s) |
Liang J, Lin Y |
| Citation missing |
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| Submission date |
Nov 05, 2007 |
| Last update date |
Oct 01, 2021 |
| Contact name |
Jian-Jong Liang |
| E-mail(s) |
jjliang1234@yahoo.com.tw
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| Phone |
886-2-2789-9013
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| Organization name |
Academia Sinica
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| Department |
Institute of Biomedical Sciences
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| Lab |
443
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| Street address |
No.128 Academia Road, Section 2
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| City |
Taipei |
| State/province |
Taiwan |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA103331 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9524_RAW.tar |
8.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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