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| Status |
Public on Jun 01, 2017 |
| Title |
Innate versus adaptive lymphoid cell fate choice is controlled by the E-Id protein axis [ATAC-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The lymphoid branch of the immune defense is composed of innate and adaptive immune cells. Using multiple genetic strategies we demonstrate that in the thymus E2A and HEB act in synergy to establish T cell identity and to suppress the aberrant development of innate lymphoid cells that include ILC2 and LTi-like cells. We found that E2A and HEB induce T cell fate by activating the expression of an ensemble of genes encoding for proteins associated with Notch- and pre-TCR signaling and to promote TCRβ antigen receptor assembly. We show that E2A and HEB act in early T progenitors (ETPs) to establish and maintain a T-lineage specific enhancer repertoire, including regulatory elements associated with the Notch1/3 and Rag1/2 gene loci. Based on these and previous observations we propose that the E-Id protein axis specifies innate versus adaptive lymphoid cell fate.
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| Overall design |
Examination of transcriptome and open chromatin regions in wild-type and E2Afl/flHEBfl/flIL7RCre ETPs by RNA-seq and ATAC-seq.
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| Contributor(s) |
Miyazaki M, Lin YC, Murre C |
| Citation(s) |
28514688 |
| |
| Submission date |
Feb 24, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Kazuko Miyazaki |
| E-mail(s) |
kamiyazaki@infront.kyoto-u.ac.jp
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| Organization name |
Institute for Life and Medical Sciences, Kyoto University
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| Lab |
Department of Immunology
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| Street address |
53 Kawahara-cho, Shogoin, Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE95339 |
Innate versus adaptive lymphoid cell fate choice is controlled by the E-Id protein axis |
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| Relations |
| BioProject |
PRJNA376694 |
| SRA |
SRP100677 |
