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| Status |
Public on Nov 20, 2017 |
| Title |
Single cell RNA-seq of eight thousand epithelium cells from the mammary glands of adult mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Epithelial cells were isolated by FACS from the mammary glands of adult (10 week old) female mice. A basal subpopulation of the epithelial cells was also isolated. Freshly sorted cells were submitted to a 10X Genomics Chromium System for single cell capture. cDNA synthesis and library preparation was done according to the protocol supplied by the manufacturer. Sequencing was carried out on an Illumina NextSeq500 sequencer to achieve 75 bp paired-end reads.
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| Overall design |
Transcriptional profiling was completed for 4771 basal cells and 3302 total epithelial cells from 8 mice.
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| Contributor(s) |
Smyth GK, Chen Y, Pal B, Visvader JE |
| Citation(s) |
29158510, 37994351 |
| |
| Submission date |
Feb 27, 2017 |
| Last update date |
Dec 07, 2023 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE103275 |
Single cell RNA-seq of mouse mammary gland epithelium cells |
|
| Relations |
| BioProject |
PRJNA377105 |
| SRA |
SRP100782 |
