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Series GSE95430 Query DataSets for GSE95430
Status Public on Nov 20, 2017
Title Single cell RNA-seq of eight thousand epithelium cells from the mammary glands of adult mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Epithelial cells were isolated by FACS from the mammary glands of adult (10 week old) female mice. A basal subpopulation of the epithelial cells was also isolated. Freshly sorted cells were submitted to a 10X Genomics Chromium System for single cell capture. cDNA synthesis and library preparation was done according to the protocol supplied by the manufacturer. Sequencing was carried out on an Illumina NextSeq500 sequencer to achieve 75 bp paired-end reads.
Overall design Transcriptional profiling was completed for 4771 basal cells and 3302 total epithelial cells from 8 mice.
Contributor(s) Smyth GK, Chen Y, Pal B, Visvader JE
Citation(s) 29158510, 37994351
Submission date Feb 27, 2017
Last update date Dec 07, 2023
Contact name Gordon K Smyth
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM2510616 Adult basal cells
GSM2510617 Adult epithelial cells
This SubSeries is part of SuperSeries:
GSE103275 Single cell RNA-seq of mouse mammary gland epithelium cells
BioProject PRJNA377105
SRA SRP100782

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Supplementary file Size Download File type/resource
GSE95430_RAW.tar 20.5 Mb (http)(custom) TAR (of BAI, MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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