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| Status |
Public on Jul 24, 2017 |
| Title |
Transcriptome-wide identification of novel UV-B- and light modulated flavonol pathway genes controlled by VviMYBF1 |
| Organism |
Vitis vinifera |
| Experiment type |
Expression profiling by array
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| Summary |
Flavonols constitute a group of flavonoids with important photoprotective roles in plants. In addition, flavonol content and composition greatly influences fruit quality. We previously demonstrated that the grapevine R2R3-MYB transcription factor VviMYBF1 promotes flavonol accumulation by inducing the expression of flavonol synthase (VviFLS1/VviFLS4), a key step of the initial flavonol pathway. Despite this, gene networks underlying flavonol modification in grapevine including both structural and regulatory genes remain poorly understood. In order to identify flavonol modifying genes and transcription factors acting downstream of VviMYBF1 a microarray-based transcriptome analysis was performed on grapevine hairy roots ectopically expressing VviMYBF1 or a Green Fluorescent Protein as control. VviFLS1 was induced in VviMYBF1 transgenic roots and flavonols, mainly quercetin 3-glucoside and quercetin 3-galactoside, accumulated significantly compared with control lines. Among the differentially expressed genes, potential flavonol-modifying enzymes with predicted rhamnosyltransferase (e.g. RhaT1) or glycosyltransferase (e.g. GT3) activities were identified. In addition, important transcription factors of the MYB and bZIP families such as the proanthocyanidin regulator VviMYBPA1 and the UV-B light responsive HY5 homologue VviHYH were significantly altered in their expression pattern by overexpression of VviMYBF1. In silico promoter sequence analysis confirmed that the significantly differentially expressed genes contained binding sites similar to known MYB recognition elements. Co-temporal expression analysis demonstrated positive correlation of VviMYBF1 and accumulation of glycosylated flavonols with VviGT3 and VviRhaT1 during berry development and in fruits ripened with different light and UV-B radiation conditions at field. These results show that VviMYBF1 overexpression led to the identification of novel genes of the flavonol pathway and that the flavonol modifying machinery can be influenced by agricultural practices to optimize flavonol composition in grapes.
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| Overall design |
Transgenic hairy roots were generated by infiltration of Vitis vinifera cv. ‘Chardonnay’ leaves with Agrobacterium rhizogenes carrying either VviMYBF1 or GFP (control) cDNA. To analyze differential gene expression between VviMYBF1 and GFP control hairy roots, total RNA was extracted from stable transgenic lines and subsequent array hybridization and microarray analysis was performed using the custom Affymetrix GrapeGen chip.
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| Contributor(s) |
Czemmel S, Bogs J |
| Citation(s) |
28690624 |
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| Submission date |
Feb 28, 2017 |
| Last update date |
Jul 25, 2017 |
| Contact name |
Stefan Czemmel |
| Organization name |
Quantitative Biology Center (QBiC)
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| Department |
University of Tuebingen
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| Street address |
Auf der Morgenstelle 10
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| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
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| Platforms (1) |
| GPL11004 |
Affymetrix GrapeGen Vitis vinifera Microarray [CDF: GrapeGena520510F] |
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| Samples (7)
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| GSM2515907 |
grapevine hairy root line, transgenic for VviMYBF1,sample 32,biological replicate 1 |
| GSM2515908 |
grapevine hairy root line, transgenic for VviMYBF1,sample 33,biological replicate 2 |
| GSM2515909 |
grapevine hairy root line, transgenic for VviMYBF1,sample 34,biological replicate 3 |
| GSM2515910 |
grapevine hairy root line, transgenic for VviMYBF1,sample 62,biological replicate 4 |
| GSM2515911 |
grapevine hairy root line, transgenic for GFP,sample 77,biological replicate 1 |
| GSM2515912 |
grapevine hairy root line, transgenic for GFP,sample 83,biological replicate 2 |
| GSM2515913 |
grapevine hairy root line, transgenic for GFP,sample 97,biological replicate 3 |
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| Relations |
| BioProject |
PRJNA377333 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE95532_RAW.tar |
19.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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