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Series GSE95689 Query DataSets for GSE95689
Status Public on Jun 06, 2017
Title Widespread changes in nucleosome accessibility without changes in nucleosome occupancy during a rapid transcriptional induction
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Activation of transcription requires alteration of chromatin by complexes that increase the accessibility of nucleosomal DNA. Removing nucleosomes from regulatory sequences has been proposed to play a significant role in activation. We tested whether changes in nucleosome occupancy occurred on the set of genes that are activated by the unfolded protein response (UPR). We observed no decrease in occupancy on most promoters, gene bodies, and enhancers. Instead there was an increase in the accessibility of nucleosomes, as measured by MNase digestion and by ATAC-seq, that did not result from removal of the nucleosome. Thus changes in nucleosome accessibility predominate over changes in nucleosome occupancy during rapid transcriptional induction during the UPR.
 
Overall design Time series of nucleosome occupancy, MNase-seq, ATAC-seq, H3K27ac, RNAseq and Pol2 profiles for UPR-induced S2 cells.
 
Contributor(s) Mueller B, Mieczkowski J, Kundu S, Wang P, Sadreyev R, Tolstorukov MY, Kingston RE
Citation(s) 28356342
Submission date Mar 04, 2017
Last update date May 15, 2019
Contact name Michael Tolstorukov
E-mail(s) tolstorukov@molbio.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Molecular Biology
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platforms (2)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (64)
GSM2521696 S2 T0 H3 ChIP rep1 1.5U MNase
GSM2521697 S2 T0 H3 ChIP rep1 6.25U MNase
GSM2521698 S2 T0 H3 ChIP rep1 25U MNase
Relations
BioProject PRJNA377957
SRA SRP101384

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Supplementary file Size Download File type/resource
GSE95689_RAW.tar 2.5 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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