|
| Status |
Public on Nov 12, 2008 |
| Title |
P. aeruginosa PA14-WT vs pyrF mutant vs pyrF mutant w/uracil |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
Comparative transcriptome analyses of P. aeruginosa PA14 pyrF mutant with PA14 wild type, and with PA14 pyrF mutant with 1 mM uracil supplement and PA14 wild type with 10 mM uracil in biofilm cells. All samples were cultured in LB with glass wool at 37C for 7h.
Keywords: Pseudomonas aeruginosa biofilm pyrF uracil
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| |
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| Overall design |
Strains: P. aeruginosa PA14 wild type, PA14 pyrF mutant
Medium: LB for PA14 wild type and PA14 pyrF mutant, LB with 1 mM uracil for PA14 pyrF mutant, LB with 10 mM uracil for PA14 wild type
Cell type: Biofilm cells grown on glass wool
Time: 7 h
Temperature: 37C
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| |
|
| Contributor(s) |
Ueda A, Wood TK |
| Citation |
Akihiro Ueda, Can Attila, Marvin Whiteley, Thomas K. Wood. Uracil influences quorum sensing and biofilm formation in Pseudomonas aeruginosa and fluorouracil is an antagonist; Volume 2 Issue 1, Pages 62 - 74. DOI: 10.1111/j.1751-7915.2008.00060.x
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| |
| Submission date |
Nov 13, 2007 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Akihiro Ueda |
| E-mail(s) |
akihiro.ueda@chemail.tamu.edu
|
| Phone |
979-845-1744
|
| Fax |
979-845-6446
|
| Organization name |
Texas A&M University
|
| Department |
Chemical Engineering
|
| Street address |
3122 TAMU
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843-3122 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
|
| Samples (4)
|
| GSM318716 |
PA14 WT w/10 mM uracil LB 37C 7h biofilms |
|
| Relations |
| BioProject |
PRJNA103433 |
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