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| Status |
Public on Oct 04, 2017 |
| Title |
Cytotoxic effects of radiocontrast dyes on human umbilical cord mesenchymal stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Discography is an imaging procedure typically performed on patients who report extended periods of pain.Usually radiocontrast agents are used to diagnose the discogenic low back pain.
This study was focused on investigating the effects of two clinically used, non-ionic radiocontrast dyes, isovue-370 and omnipaque-240, on the human umbilical cord mesenchymal stem cells (UC-MSCs). Human UC-MSCs were cultured and treated with various concentrations of Omnipaque and Isovue for 24 hr. The cell growth was inhibited by both the dyes.
The results showed that both the dyes significantly affected the cell morphology and viability as well as caused apoptosis in UC-MSCs. The global gene expression analysis showed that the most effected genes were those involved in the apoptosis, DNA damage and toxicity.
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| Overall design |
Human umbilical cord derived MSCs were cultured in vitro and treated with 1:4 (dye:medium) of either Omnipaqe or Isovue for 24 hrs. Gene expression of treated cells was compared to untreated cultured cells.
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| Contributor(s) |
Beeravolu N, McKee C, Perez-Cruet M, Thibodeau BJ, Wilson GD, Chaudhry R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 09, 2017 |
| Last update date |
Oct 06, 2017 |
| Contact name |
Bryan Thibodeau |
| E-mail(s) |
bryan.thibodeau@beaumont.edu
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| Phone |
248-551-0275
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| Organization name |
Beaumont Research Institute
|
| Department |
BioBank
|
| Street address |
3811 West 13 Mile Road
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| City |
Royal Oak |
| State/province |
MI |
| ZIP/Postal code |
48073 |
| Country |
USA |
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| Platforms (1) |
| GPL10904 |
Illumina HumanHT-12 V4.0 expression beadchip (gene symbol) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA378653 |
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