|
| Status |
Public on Mar 01, 2018 |
| Title |
Genome Scale Analysis of miRNA and mRNA regulation during preterm labor [whole blood] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to define relationships between peripheral blood miRNAs and mRNAs of women undergoing idiopathic preterm labor (PTL) and compare network level changes to control women that deliver at term.Using RNA Sequencing we have performed global miRNA and mRNA profiling in both monocytes and whole blood leukocytes of women who underwent PTL (N=15) matched to non-pathological controls (N=30) as a part of the Ontario Birth Study cohort. We have identified differentially expressed miRNAs, mRNAs and pathways associated with PTL. Intriguingly, we found perturbations in many cellular signaling pathways, particularly in interleukin signaling. We also predicted mRNA targets for specific miRNAs and used these predictions to build putative miRNA-mRNA networks. We identified 6 miRNAs significantly associated with PTL whose expression is negatively correlated with expression of 14 predicted mRNA targets that are also significantly associated with PTL.
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| |
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| Overall design |
miRNA and mRNA were quantified from whole blood and monocytes of women undergoing spontaneous preterm labor compared to nonlabor controls matched on gestational age
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| |
|
| Contributor(s) |
Lye S, Price N |
| Citation(s) |
29305255 |
| |
| Submission date |
Mar 10, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
alison g paquette |
| E-mail(s) |
ali.paquette@gmail.com
|
| Organization name |
Institute of Systems Biology
|
| Lab |
Price
|
| Street address |
401 Terry Avenue N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (38)
|
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| This SubSeries is part of SuperSeries: |
| GSE96097 |
Genome Scale Analysis of miRNA and mRNA regulation during preterm labor |
|
| Relations |
| BioProject |
PRJNA378794 |
| SRA |
SRP101737 |
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