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| Status |
Public on Sep 18, 2017 |
| Title |
The splicing co-factor Barricade/Tat-SF1, is required for cell cycle and lineage progression in Drosophila neural stem cells. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Stem cells need to balance self-renewal and differentiation for correct tissue development and homeostasis. Defects in this balance can lead to developmental defects or tumor formation. In recent years, mRNA splicing has emerged as one important mechanism regulating cell fate decisions. Here we address the role of the evolutionary conserved splicing co-factor Barricade (Barc)/CUS2/Tat-SF1 in Drosophila neural stem cell (neuroblast) lineage formation. We show that Barc is required for the generation of neurons during Drosophila brain development by ensuring correct neural progenitor proliferation and differentiation. Barc associates with components of the U2 small nuclear ribonucleic proteins (snRNP), and its depletion causes alternative splicing in form of intron retention in a subset of genes. Using bioinformatics analysis and a cell culture based splicing assay, we found that Barc dependent introns share three major traits: they are short, GC rich and have weak 3’ splice sites. Our results show that Barc, t...
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| Overall design |
Comparison of transcriptomes of Drosophila melanogaster wild-type and barc RNAi neural stem cells (type I) and S2 cells.
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| Contributor(s) |
Abramczuk MK, Burkard TR, Knoblich JA |
| Citation(s) |
28935704 |
| |
| Submission date |
Mar 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Thomas Rainer Burkard |
| E-mail(s) |
thomas.burkard@imba.oeaw.ac.at
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| Organization name |
IMBA
|
| Street address |
Dr. Bohrgasse 7
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
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| Platforms (2) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (12)
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| GSM2535382 |
wild type larval type I neuroblasts - replicate 1 |
| GSM2535383 |
wild type larval type I neuroblasts - replicate 2 |
| GSM2535384 |
barc RNAi larval type I neuroblasts - replicate 1 |
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| Relations |
| BioProject |
PRJNA378990 |
| SRA |
SRP101797 |
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