 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 19, 2017 |
| Title |
Time-dependent regulation of cellular programming of monocytes by NCOR2 [RNASeq_KD] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Whole transcriptome profiling (RNA-Seq) was performed on human Mo-GMCSF[IL4 (0-72h)] cells with either NCOR2 being knocked down or corresponding WT cells
|
| |
|
| Overall design |
CD14+ monocytes were FACS-sorted from blood of human healthy donors and later activated in vitro with the combination of GMCSF and IL4 for 72h to obtain Mo-GMCSF[IL4 (0-72h)] cells. During the last 24 hours of activation, either siRNAs targeting NCOR2 or scrambled RNAs were added to obtain NCOR2 knock down cells and corresponding WT cells, respectively
|
| |
|
| Contributor(s) |
Sander J, Schmidt SV, Cirovic B, McGovern N, Papantonopoulou O, Hardt A, Aschenbrenner A, Kreer C, Quast T, Xu A, Schmidleithner LM, Theis H, Do L, Lauterbach M, Schulte-Schrepping J, Günther P, Xue J, Baßler K, Ulas T, Klee K, Herresthal S, Krebs W, Martin B, Latz E, Händler K, Kraut M, Kolanus W, Beyer M, Falk C, Burgdorf S, Melosh NA, Ginhoux F, Newell E, Schlitzer A, Schultze JL |
| Citation(s) |
29262348 |
| |
| Submission date |
Mar 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE96719 |
Time-dependent regulation of cellular programming of monocytes by NCOR2 |
|
| Relations |
| BioProject |
PRJNA379498 |
| SRA |
SRP102092 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE96713_GEO_Schultze_RNASeq_KD_processed_NORM_FILT.txt.gz |
276.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...