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| Status |
Public on Mar 20, 2017 |
| Title |
Comparative gene expression analysis of Porphyromonas gingivalis ATCC 33277 in planktonic and biofilm states |
| Organism |
Porphyromonas gingivalis |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of the present study was to compare the genome-wide transcription activities of P.gingivalis under both states, as biofilm and as planktonic growth, using microarray technology.
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| Overall design |
P. gingivalis ATCC 33277 was incubated in multiwell culture plates at 37ºC for 96 hours in anaerobic conditions using an in vitro static model to to develop both the planktonic and biofilm states (the latter over sterile ceramic calcium hydroxyapatite discs). After incubation, the bacterial cells were harvested and total RNA was extracted, treated with DNase-I, and purified. Three biological replicates for each cell state were independently hybridized for transcriptomic comparison.
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| Contributor(s) |
Romero-Lastra P, Sanchez MC, Vidal H, Llama-Palacios A, Figuero E, Herrera D, Sanz M |
| Citation(s) |
28369099 |
| |
| Submission date |
Mar 17, 2017 |
| Last update date |
Jun 19, 2017 |
| Contact name |
Patricia Romero Lastra |
| E-mail(s) |
patrrome@ucm.es
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| Organization name |
Universidad Complutense de Madrid
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| Street address |
Plaza de Ramon y Cajal s/n
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| City |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
| GPL23193 |
Agilent_074976 Porphyromonas gingivalis Microarray |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA379578 |
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