|
| Status |
Public on Jan 25, 2018 |
| Title |
Weighted gene coexpression analysis of RNA-seq data from 65d juvenile Area X and adjacent non-song ventral striatopallidum (VSP). |
| Organism |
Taeniopygia guttata |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Human speech is one of the few examples of vocal learning among mammals, yet ~half of avian species exhibit this ability. Its genetic basis is unknown beyond a shared requirement for FoxP2 in both humans and zebra finches. Here we manipulated FoxP2 isoforms in Area X during a critical period for song development, delineating, for the first time, unique contributions of each to vocal learning. We used weighted gene coexpression network analysis of RNA-seq data to construct transcriptional profiles and found gene modules correlated to singing, learning, or vocal variability. The juvenile song modules were preserved adults, whereas the learning modules were not. The learning modules were preserved in the striatopallidum adjacent to Area X whereas the song modules were not. The confluence of learning and singing coexpression in juvenile, but not adult, Area X may underscore molecular processes that drive vocal learning in zebra finches and, by analogy, humans.
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| Overall design |
19 samples each from Area X and VSP for birds injected in Area X with AAV1 to overexpress GFP, full length FoxP2, or truncated FoxP2. Biological replicates but not technical replicates are included.
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| Contributor(s) |
Burkett ZD, Day NF, Kimball TH, Aamodt CM, Heston JB, Hilliard AT, Xiao X, White SA |
| Citation(s) |
29360038 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 MH070712 |
Formation and Function of Circuitry for Vocal Learning |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
STEPHANIE ANN WHITE |
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| |
| Submission date |
Mar 21, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Zachary Daniel Burkett |
| E-mail(s) |
zburkett@ucla.edu
|
| Phone |
310-267-4017
|
| Organization name |
University of California, Los Angeles
|
| Department |
Integrative Biology & Physiology
|
| Lab |
White Lab
|
| Street address |
610 Charles E Young Drive East Room 1045
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90024 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL23198 |
Illumina HiSeq 2500 (Taeniopygia guttata) |
|
| Samples (38)
|
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| Relations |
| BioProject |
PRJNA379914 |
| SRA |
SRP102220 |
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