NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE96922 Query DataSets for GSE96922
Status Public on Mar 27, 2017
Title Histone gene replacement reveals a post-transcriptional role for H3K36 in maintaining metazoan transcriptome fidelity
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
Summary To study the function of this residue independent from the enzymes that modify it, we used a “histone replacement” system in Drosophila to generate a non-modifiable H3K36 lysine-to-arginine (H3K36R) mutant. Post-translational modification of histone H3K36 is neither required to suppress cryptic transcription initiation nor to include alternative exons in Drosophila; instead it promotes expression of active genes by stimulating polyadenylation.
 
Overall design Examination of poly-A RNA-seq, total nuclear RNA-seq, Start-seq, and ATAC-seq from HWT and K36R rescue larvae
 
Contributor(s) Meers M, Matera AG
Citation(s) 28346137, 29466941
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 DA036897 Engineering histone genes to interrogate the epigenetic code in space and time UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL A. Gregory MATERA
Submission date Mar 22, 2017
Last update date May 23, 2019
Contact name Michael Meers
E-mail(s) mpmeers@email.unc.edu
Organization name UNC Chapel Hill
Street address 250 Bell Tower Dr.
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (3)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
Samples (52)
GSM2545903 HWT Poly-A RNA-seq rep1
GSM2545904 HWT Poly-A RNA-seq rep2
GSM2545905 HWT Poly-A RNA-seq rep3
Relations
BioProject PRJNA380106
SRA SRP102296

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE96922_HWT.ATAC.accepted_hits.sorted.hiquality.RPKM.bw 12.8 Mb (ftp)(http) BW
GSE96922_K36R.ATAC.accepted_hits.sorted.hiquality.RPKM.bw 13.1 Mb (ftp)(http) BW
GSE96922_dm3.nuc.DESeq2.diff.gz 773.0 Kb (ftp)(http) DIFF
GSE96922_dm3.polyA.DESeq2.diff.gz 740.6 Kb (ftp)(http) DIFF
GSE96922_nuTSS_calls.full.header.bed.gz 552.9 Kb (ftp)(http) BED
GSE96922_obsTSS_calls.full.bed.gz 1.2 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap