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| Status |
Public on Mar 24, 2017 |
| Title |
The sea urchin larval response to immune challenge with Vibrio diazotrophicus |
| Organism |
Strongylocentrotus purpuratus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Seawater exposure to the gram negative marine bacterium Vibrio diazotrophicus induces a robust cellular response in sea urchin larvae that includes the migration of pigment cells to the gut epithelium, changes in cell behavior and altered gut morphology (Ho et al., 2016; PMID 27192936). To investigate the transcriptional underpinnings of this response, whole transcriptome sequencing was performed on mRNA isolated from larval samples collected at 0, 6, 12 and 24 hr of exposure to V. diazotrophicus. The morphological simplicity of the sea urchin larva provides a systems-level model for identifying biologically relevant transcriptional state changes in response to dysbiosis in the gut lumen.
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| Overall design |
Sea urchin larvae were sampled at four time points in response to bacterial exposure
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| Contributor(s) |
Buckley KM, Rast JP |
| Citation missing |
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| |
| Submission date |
Mar 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Katherine Michele Buckley |
| E-mail(s) |
kbuckley@gmail.com
|
| Organization name |
The George Washington University
|
| Department |
Department of Biological Sciences
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| Street address |
800 22nd St NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20052 |
| Country |
USA |
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| Platforms (1) |
| GPL23220 |
AB SOLiD 4 System (Strongylocentrotus purpuratus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA380184 |
| SRA |
SRP102343 |
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