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| Status |
Public on Mar 30, 2017 |
| Title |
Genome-wide multi-omics profiling reveals extensive genetic complexity in 8p11-p12 amplified breast carcinomas [expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptomic profiling of human breast tumors.
Genomic and expression profiling using 38K BAC array-CGH and Illumina HT-12 beadchips were performed on 53 invasive breast tumors to assess the impact of gene dosage on gene expression patterns and the effect of other mechanisms on transcriptional levels. Array-CGH results was validated by FISH using tumors showing 8p11-p12 DNA amplification and expression profiling was confirmed using qPCR for 11 transcripts. Low-level gain, high-level gain/amplification, heterozygous loss and homozygous deletion (henceforth referred to as gain, amplification, loss and deletion) were defined as log2 ratio thresholds set at +0.2, >= +0.5, -0.2 and <=-1.0, respectively.
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| Overall design |
Total RNA was isolated from fresh-frozen tissue samples
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| Contributor(s) |
Parris TZ, Rönnerman EW, Engqvist H, Biermann J, Truvé K, Nemes S, Forssell-Aronsson E, Kovács A, Karlsson P, Helou K |
| Citation(s) |
20551037, 24662924, 28877888, 29844878, 35331169, 36123629 |
| |
| Submission date |
Mar 29, 2017 |
| Last update date |
Oct 04, 2022 |
| Contact name |
Toshima Z. Parris |
| E-mail(s) |
toshima.parris@oncology.gu.se
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| Organization name |
University of Gothenburg
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| Department |
Department of Oncology
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| Lab |
Sahlgrenska Cancer Center
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| Street address |
Medicinaregatan 1G, 6th floor
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| City |
Gothenburg |
| ZIP/Postal code |
41390 |
| Country |
Sweden |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (53)
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| This SubSeries is part of SuperSeries: |
| GSE97293 |
Genome-wide multi-omics profiling reveals extensive genetic complexity in 8p11-p12 amplified breast carcinomas |
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| Relations |
| BioProject |
PRJNA380897 |
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