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| Status |
Public on Apr 08, 2017 |
| Title |
Exposure of bovine oocytes to elevated insulin concentration (10 ug/ml) during in vitro maturation changes DNA methylation pattern in Day-8-blastocysts – an epigenetic study. |
| Organism |
Bos taurus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
The aim was to study the potential effect of hyperinsulinemia on gene expression and differential DNA-methylation of cow embryos by adding 10 µg/ml (INS10) during in vitro oocyte maturation.
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| Overall design |
In total, 882 abattoir-derived oocytes (equally distributed between both treatments (INS10) and a control, (INS0)) produced 199 embryos in a period of 8 weeks. Cleavage blastocyst rates were recorded and embryos evaluated for stage and quality grade before individually frozen at -80 °C. 120 of the embryos were used for the methylome analysis, pooled in groups of 10 embryos with a similar distribution of embryos (pools adjusted for quality grade and developmental stage in order to avoid confounding effects in the results due to different embryo characteristics). The experiment was run in four replicates per treatment group.
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| Contributor(s) |
Laskowski D, Humblot P, Sirard M, Sjunnesson Y, Båge R, Andersson G |
| Citation(s) |
29745447 |
| Submission date |
Apr 07, 2017 |
| Last update date |
Nov 12, 2018 |
| Contact name |
Marc-André Sirard |
| E-mail(s) |
Marc-Andre.Sirard@fsaa.ulaval.ca
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| Organization name |
Université Laval
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| Department |
Sciences Animales
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| Street address |
Offfice 2732, 2440 Hochelaga Blvd.
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| City |
Québec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
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| Platforms (1) |
| GPL18384 |
EmbryoGENE DNA Methylation Array Bovine 2x400K v2.0 |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE97517 |
Exposure of bovine oocytes to insulin concentration (0.1 and 10 ug/ml) during in vitro maturation changes DNA methylation pattern in Day-8-blastocysts - an epigenetic study |
|
| Relations |
| BioProject |
PRJNA382157 |
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