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| Status |
Public on Dec 01, 2022 |
| Title |
Identification of Bacillus anthracis genes differentially expressed in human serum |
| Platform organism |
Bacillus anthracis |
| Sample organism |
Bacillus anthracis str. Sterne |
| Experiment type |
Expression profiling by array
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| Summary |
Bacillus anthracis causes anthrax infections in mammals. Large-scale mortality resulting from the intentional release of B. anthracis spores represents a potential bioterrorism threat. Inhalational anthrax almost invariably proceeds to fatal systemic infection, characterized by massive bacteremia. A better understanding of host-pathogen interactions is urgently needed for effective treatment of this lethal disease. However, virulence mechanisms used by B. anthracis to survive and multiply in human blood are not completely understood. Identification of genes that are differentially expressed during the growth of B. anthracis in human serum can elucidate how this pathogen successfully colonizes the bloodstream. We compared the transcriptional profile of B. anthracis growing in heat-inactivated human serum to that in LB medium. Genes involved in the biosynthesis of purines, certain amino acids and riboflavin and lipid metabolism, genes encoding ABC transporters, respiratory enzymes and several genes with hypothetical function were identified as being upregulated during growth in serum.
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| Overall design |
Direct comparison of experimental (human serum) RNA samples to control (LB) RNA samples in a two-color hybridization. Four independent experimental samples were compared against four indepdent control samples with dye-swapping.
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| Contributor(s) |
Samant S, Lee H |
| Citation missing |
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| |
| Submission date |
Apr 10, 2017 |
| Last update date |
Dec 03, 2022 |
| Contact name |
Hyunwoo Lee |
| E-mail(s) |
hlee31@uic.edu
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Center for Biomolecular Sciences
|
| Street address |
900 S. Ashland Ave., MBRB #3020
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60607 |
| Country |
USA |
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| Platforms (1) |
| GPL23273 |
JCVI PFGRC Bacillus anthracis 27K v2 array designed primarily based on strain Ames |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA382329 |
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