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Series GSE97666 Query DataSets for GSE97666
Status Public on Oct 21, 2020
Title RNA-seq analysis of regulatory T cells in thymus, placenta, spleen and white adipose tissue of pregnant mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In this study we performed RNA sequencing to determine and compare the transcriptome of regulatory T cells (Tregs) present in the thymus, placenta, spleen and white adipose tissue (FAT) of pregnant mice. We further assessed how the expression of the receptor Rank in the thymic medullary epithelia affects the transcriptional program of these Treg populations. For the isolation of viable and bona fide Tregs, we first generated RankWt (wild type control mice) and RankΔFoxn1 (which lack expression of Rank in the thymic epithelia) and further crossed them to mice that express GFP from the Foxp3 promoter. Neuropilin1 was used to mark the thymic origin of the Tregs. Equal numbers of Tregs (280 cells) were sorted as CD45-CD8-CD4+GFP+Neuropilin1High cells from the thymus and placenta of RankWtFoxp3GFP/GFP and RankΔFoxn1Foxp3GFP/GFP pregnant females at E17.5 (both samples from the same female; n=4 females per genotype). Thymus Tregs from non-pregnant littermate female mice for each genotype cohort were also studied. To increase robustness, the 280 placental Tregs were purified from 5 individual placentas per pregnant female (56 Tregs/placenta). Our study is the first one to determine the transcriptome of thymus and placenta Tregs during pregnancy and reveals that, partially dependent on Rank expression in the thymic epithelia, placental-resident Tregs are molecularly distinct from thymic Tregs. In a separate experiment with different pregnant mice than those used for thymus and placenta analysis, VAT Tregs (20-30 cells) and splenic Tregs (150 cells) were sorted (both samples from the same female) by using the same mouse lines (Rank Wt and RankΔFoxn1), embryological days of analysis (E17.5) and sorting strategies.
Overall design RNA-seq analysis of small, FACS-sorted cell pools.

Affiliation: Magdalena Paolino, IMBA - Institute of Molecular Biotechnology of the Austrian Academy of Sciences

Affiliation: Josef M. Penninger, IMBA - Institute of Molecular Biotechnology of the Austrian Academy of Sciences

Contributor(s) Paolino M, Penninger JM
Citation(s) 33361811
Submission date Apr 11, 2017
Last update date Mar 02, 2021
Contact name Christoph Bock
Organization name CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code 1090
Country Austria
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (34)
GSM2574898 GTT11_P
GSM2574899 GTT11_T
GSM2574900 GTT12_P
BioProject PRJNA382592
SRA SRP103818

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE97666_rnaseq_deseq_global_woo_counts_raw.tsv.gz 2.2 Mb (ftp)(http) TSV
GSE97666_rnaseq_deseq_global_woo_fpkms.tsv.gz 6.7 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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