|
| Status |
Public on Jun 20, 2017 |
| Title |
The Conserved RNA Binding Cyclophilin, Rct1, Regulates Small RNA Biogenesis and Splicing Independent of Heterochromatin Assembly [ChIP-seq] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
RNAi factors and their catalytic activities are essential for heterochromatin assembly in S. pombe. This has led to the idea that siRNAs can promote H3K9 methylation by recruiting the Cryptic Loci Regulator Complex (CLRC), also known as Recombination In K Complex (RIKC), to the nucleation site. The conserved RNA-binding protein Rct1 (AtCyp59/SIG-7) interacts with splicing factors and with RNA polymerase II. Here we show that Rct1 promotes processing of pericentromeric transcripts into siRNAs, via the RNA recognition motif. Surprisingly, loss of siRNA in rct1 mutant cells has no effect on H3K9 di- or tri-methylation, resembling other splicing mutants, and suggesting post-transcriptional gene silencing per se is not required to maintain heterochromatin. Splicing of the Argonaute gene is also defective in rct1 mutants, and contributes to loss of silencing but not to loss of siRNA. Our results suggest that Rct1 guides transcripts to the RNAi machinery by promoting splicing of elongating non-coding transcripts.
|
| |
|
| Overall design |
ChIP-seq of H3K9me2, H3K9me3, nonspecific RNAPII (8WG16), initiating RNAPII (phospho S5), and elongating RNAPII (phospho S2). Whole cell extract (DNA input) samples are included for each experiment. 38 samples in total.
|
| |
|
| Contributor(s) |
Chang A, Castel SE, Ernst E, Kim HS, Martienssen RA |
| Citation(s) |
28636937 |
| |
| Submission date |
Apr 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Robert A Martienssen |
| E-mail(s) |
martiens@cshl.edu
|
| Organization name |
Cold Spring Harbor Laboratory
|
| Department |
Delbruck Bldg.
|
| Lab |
Martienssen
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
|
| Samples (38)
|
|
| This SubSeries is part of SuperSeries: |
| GSE97749 |
The Conserved RNA Binding Cyclophilin, Rct1, Regulates Small RNA Biogenesis and Splicing Independent of Heterochromatin Assembly |
|
| Relations |
| BioProject |
PRJNA382811 |
| SRA |
SRP103867 |
Less...
More...