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| Status |
Public on Jun 26, 2018 |
| Title |
Expression profile of hematopoietic stem and progenitor cells (HSPCs) after conditional deletion of the histone 3 lysine 4 (H3K4) methyltransferase Setd1b in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Loss of Setd1b in adult mice using both ubiquitous and hematopoietic-specific deletion strategies caused lethality arising from severe hematopoietic defects. To identify genes regulated by Setd1b in HSPCs we performed RNA-seq analysis of the LSK (Lin-, Sca+, Kit+) compartment in the bone marrow. Mice carrying heterozygous and homozygous conditional Setd1b alleles and Rosa26-Cre-ERT2 were treated with Tamoxifen for 6 days (4 days gavage - 3 days pause - 2 days gavage). Mice were sacrificed 3 days after the last treatment and RNA was isolated and processed for next generation sequencing. We found that Setd1b regulates key lineage specification components including Cebpa, Gata-1 and Klf-1.
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| Overall design |
HSPC (LSK) mRNA profiles of Setd1b control (heterozygous) and knockout (homozygous) littermates were generated 3 days after the last tamoxifen administration, in triplicates.
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| Contributor(s) |
Anastassiadis K, Schmidt K |
| Citation(s) |
29916805 |
| |
| Submission date |
Apr 19, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Konstantinos Anastassiadis |
| E-mail(s) |
konstantinos.anastassiadis@tu-dresden.de
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| Phone |
+49 351 46340127
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| Organization name |
Technische Universitaet Dresden
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| Department |
Biotechnology Center
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| Lab |
Stem Cell Engineering
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| Street address |
Tatzberg 47
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| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA383551 |
| SRA |
SRP104275 |
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