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| Status |
Public on Aug 20, 2017 |
| Title |
Changes in gene expression due to alpha-crystallin mutations cryaa-R49C and cryab-R120G in mutant knock-in mouse lenses |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the relationship between histones, chaperone function, and cataracts, we performed RNA-seq, isothermal titration calorimetry (ITC), size-exclusion chromatography, and gel electrophoresis of histones. The RNA-seq of postnatal lenses from 2-day-old cryaa -R49C mice revealed increased histone gene expression, suggesting that a α-crystallin mutation regulates histones via a transcriptional mechanism .
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| Overall design |
RNA-seq studies on lenses of 2-day-old wild-type and 2-day-old cryaa-R49C heterozygous mutant and cryaa-R49C homozygous mutant knock-in mice; and 14-day old wild-type and 14-day-old cryab-R120G heterozygous mutant and cryab-R120G homozygous mutant knock-in mice
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| |
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| Contributor(s) |
Andley U |
| Citation(s) |
29338044 |
| |
| Submission date |
Apr 20, 2017 |
| Last update date |
Sep 19, 2019 |
| Contact name |
Usha Andley |
| E-mail(s) |
andley@vision.wustl.edu
|
| Phone |
314-362-7167
|
| Organization name |
Washington University School of Medicine
|
| Department |
Ophthalmology and Visual Sciences
|
| Lab |
Andley
|
| Street address |
660 S. Euclid Avenue
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA383718 |
| SRA |
SRP104440 |
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