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Series GSE98075 Query DataSets for GSE98075
Status Public on Aug 07, 2017
Title Interactions between effector-triggered immunity (ETI) and pattern-triggered immunity (PTI) in an Arabidopsis dde2 ein2 pad4 sid2 mutant
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary We observed inhibition of the hypersensitive response (a typical ETI response) by PTI signaling in an Arabidopsis quadruple mutant dde2 ein2 pad4 sid2 (quad).
Thus, we designed an experiment to see the interaction between ETI and PTI signaling at the transcriptome level.
The Arabidopsis line dde2 ein2 pad4 sid2 Ed-AvrRpt2 (quadAvrRpt2) was used (Ed-AvrRpt2, estradiol-inducible AvrRpt2 transgene).
In this plant line, ETI can be elicited by estradiol (Ed) treatment via in planta expression of AvrRpt2.
Transcriptome responses to PTI only, ETI only, and PTI+ETI together were recorded.
 
Overall design quadAvrRpt2 was treated with one of (1) Mock, (2) flg22 to elicit PTI response only, (3) Ed to elicit ETI response only via AvrRpt2, (4) flg22+Ed to elicit both PTI and ETI responses together.
At 0 (untouched), 60, 120, 180, and 300 minutes after treatment, treated plant leaves were harvested for RNA-seq analysis.
Three independent experiments were performed (rep1-rep3).
 
Contributor(s) Katagiri F, Mase K
Citation(s) 28811287
Submission date Apr 21, 2017
Last update date May 15, 2019
Contact name Fumiaki Katagiri
E-mail(s) katagiri@umn.edu
Phone 6126245195
Organization name Univ of Minnesota
Department Dept of Plant and Microbial Biology
Street address 1500 Gortner Ave
City St Paul
State/province Minnesota
ZIP/Postal code 55108
Country USA
 
Platforms (1)
GPL17639 Illumina HiSeq 2500 (Arabidopsis thaliana)
Samples (60)
GSM2586730 quadAvrRpt2.Mock.0min.rep1
GSM2586731 quadAvrRpt2.Mock.60min.rep1
GSM2586732 quadAvrRpt2.Mock.120min.rep1
Relations
BioProject PRJNA383861
SRA SRP104760

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98075_readcount.per.gene.out.017a.csv.gz 1.4 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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