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| Status |
Public on Apr 19, 2018 |
| Title |
Non-cell autonomous Otx2 homeoprotein regulates visual cortex plasticity through Gadd45b |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A better understanding of how Otx2 regulates plasticity in the visual cortex requires that its non-cell autonomous transcription targets be identified. We dissected layer IV of the visual cortex and used RNA-sequencing to analyze gene expression at postnatal day 30 (P30) and P100 in wild-type (WT) and Otx2+/GFP heterozygotes mice. The rationale is that CP plasticity is opened at P30 in WT but not in Otx2+/GFP mice, given that genetic deletion delays CP opening (Sugiyama et al., 2008), and that the CP is closed at P100 in WT mice and not yet in Otx2+/GFP mice. Thus, genes with similar expression at P30 in Otx2+/GFP and at P100 in WT mice but with a different level of expression during the critical period (P30 in WT or P100 Otx2+/GFP mice) were considered as potential genes involved in plasticity.
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| Overall design |
Examination of genes expression at P30 and P100 in wild-type and Otx2 (+/GFP) mice. 4 mice were pooled per condition and experiment was done in duplicate.
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| Contributor(s) |
Bernard C, Kim N, Blugeon C, Jourdren L, Di Nardo A, Prochiantz A |
| Citation(s) |
29771284 |
| |
| Submission date |
Apr 26, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jessica Apulei |
| E-mail(s) |
jessica.apulei@college-de-france.fr
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| Organization name |
Collège de France
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| Street address |
11 place marcelin berthelot
|
| City |
paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA384430 |
| SRA |
SRP105298 |
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