|
Status |
Public on Dec 20, 2019 |
Title |
Transcriptional changes induced by over-expression of miR-27a-3p in C2C12 myoblasts. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Skeletal muscle has a central role in whole body metabolism with myofibers that represent its functional units. These are differentiated cells with different contraction power and metabolic traits. Faster contracting myofibers preferentially use glucose as substrate for energy production, while slower use lipids. Myofibers can plastically change phenotypic traits in response to pathophysiological stimuli. Since post-transcriptional mechanisms should administer the changes of these post-mitotic cells, we demonstrated that miR-27a-3p influences myofiber fuel availability and mitochondrial morphology. In vitro and in vivo experiments demonstrate that miR-27a-3p regulates glycogen utilization. We used microarrays to characterize the global changes in gene expression in C2C12 myoblasts due to over-expression of miR-27a-3p.
|
|
|
Overall design |
C2C12 myoblasts were transfected with miR-27a-3p mimic or a scrambled sequence, as control. Transfection efficiency was estimated of about 60%. Transfection experiments were independently repeated in 6 replicates for miR-27a-3p mimic and 4 replicates for scrambled sequence. Results were evaluated after 72 hours of transfection.
|
|
|
Contributor(s) |
Chemello F, Cagnin S, Grespi F, Martini P, Millino C, Pacchioni B, Romualdi C, Scorrano L, Lanfranchi G |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Apr 28, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Gerolamo Lanfranchi |
E-mail(s) |
stefano.cagnin@unipd.it
|
Phone |
+39-0498276219
|
Organization name |
University of Padova
|
Department |
CRIBI - Biotechnology Center and Biology Department
|
Lab |
Functional Genomics Lab
|
Street address |
Via U. Bassi, 58/B
|
City |
Padova |
ZIP/Postal code |
35131 |
Country |
Italy |
|
|
Platforms (1) |
GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
|
Samples (10)
|
GSM2592304 |
C2C12 myoblasts transfected with miR-27a-3p rep1 |
GSM2592305 |
C2C12 myoblasts transfected with miR-27a-3p rep2 |
GSM2592306 |
C2C12 myoblasts transfected with miR-27a-3p rep3 |
GSM2592307 |
C2C12 myoblasts transfected with miR-27a-3p rep4 |
GSM2592308 |
C2C12 myoblasts transfected with miR-27a-3p rep5 |
GSM2592309 |
C2C12 myoblasts transfected with miR-27a-3p rep6 |
GSM2592310 |
C2C12 myoblasts transfected with scramble rep1 |
GSM2592311 |
C2C12 myoblasts transfected with scramble rep2 |
GSM2592312 |
C2C12 myoblasts transfected with scramble rep3 |
GSM2592313 |
C2C12 myoblasts transfected with scramble rep4 |
|
This SubSeries is part of SuperSeries: |
GSE98328 |
Expression Signatures of Single Isolated Myofibers of Mouse Hindlimb and miR-27a-3p or miR-142-3p overexpression in C2C12 myoblasts |
|
Relations |
BioProject |
PRJNA384718 |