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| Status |
Public on Jun 06, 2017 |
| Title |
Structural basis for 5’-ETS recognition by Utp4 at the early stages of ribosome biogenesis |
| Organisms |
Saccharomyces cerevisiae; Thermochaetoides thermophila |
| Experiment type |
Other
|
| Summary |
Eukaryotic ribosome biogenesis begins with the co-transcriptional assembly of the 90S pre-ribosome.
The ‘U three protein’ (UTP) complexes and snoRNA particles arrange around the nascent pre-ribosomal RNA chaperoning its folding and further maturation.
The earliest event in this hierarchical process is the binding of the UTP-A complex to the 5’-end of the pre-ribosomal RNA (5’-ETS).
This oligomeric complex predominantly consists of β-propeller and α-solenoidal proteins.
Here we present the structure of the Utp4 subunit from the thermophilic fungus Chaetomium thermophilum at 2.15 Å resolution and analyze its function by UV RNA-crosslinking (CRAC) and in context of a recent cryo-EM structure of the 90S pre-ribosome.
Utp4 consists of two orthogonal and highly basic β-propellers that perfectly fit the EM-data.
The Utp4 structure highlights an unusual Velcro-closure of its C-terminal β-propeller as relevant for protein integrity and Utp8 recognition in the context of the pre-ribosome.
We provide a first model of the 5’-ETS RNA from an internally hidden 5’-end up to the region that hybridizes to the 3’-hinge sequence of U3 snoRNA and validate a specific Utp4/5’-ETS interaction by CRAC analysis.
Altogether Utp4 is central to the UTP-A complex and organizes the 5’-ETS for further maturation.
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| Overall design |
In vitro-UV CRAC analysis
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| Contributor(s) |
Calviño FR, Kornprobst M, Schermann G, Birkle F, Wild K, Fischer T, Hurt E, Ahmed YL, Sinning I |
| Citation(s) |
28575120 |
| |
| Submission date |
Apr 28, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Ed Hurt |
| Organization name |
Biochemie Zentrum Heidelberg
|
| Department |
BZH
|
| Lab |
AG Hurt
|
| Street address |
Im Neuenheimer Feld 328
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL23386 |
Illumina HiSeq 2000 (Chaetomium thermophilum; Saccharomyces cerevisiae) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA384723 |
| SRA |
SRP105405 |
