|
| Status |
Public on Jun 29, 2017 |
| Title |
Next Generation Sequencing measuring Nod-like receptors mRNA expression in intestinal epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Intestinal epithelisal cells were obtained by EDTA isolation of ileum from WT mice followed by facs sorting (EpCAM + CD45 – 7AAD – ). Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat , and gene expression levels were measured by Cufflinks .
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| |
|
| Overall design |
Ileum epithelisal cells mRNA profiles were generated by deep sequencing, in duplicate, using illumina HiSeq 2000.
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| |
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| Contributor(s) |
Flavell RA, Zhu S |
| Citation(s) |
28636595 |
| |
| Submission date |
May 01, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Shu Zhu |
| E-mail(s) |
shu.zhu@yale.edu
|
| Organization name |
Yale University
|
| Street address |
300 Cedar Street
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA384980 |
| SRA |
SRP105771 |
