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| Status |
Public on Nov 30, 2020 |
| Title |
Transcriptional Changes in Rat Dorsal Root Ganglia Neurons During Stretch-Mediated Axon Growth |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The development of meter-long axons within the nervous system is a remarkable, yet unresolved biological process. The most widely studied aspects of neuronal growth focus on the extension and chemotropism of growth cones. However, over the course of organismal development, short axons elongate up to one meter in length with the systemic expansion of mitotic tissues. The preeminent regulatory mechanism for such synchronized growth between the nervous system and the tissues it spans, is the biomechanical stretch of axons, a known stimulus of axon growth. Here, we reveal the transcriptional profile of stretch-mediated axon growth as found using embryonic rat dorsal root ganglia neurons stretch-grown in vitro.
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| Overall design |
Computer-controlled bioreactors were used to maintain a persistent state of 25 % axon strain for one week to maximize mechanotransduced signaling changes in vitro. Genome-wide DNA microarray analysis was performed on the corresponding somata of contiguous high density axons that spanned 1.7 cm in length. Non-stretch grown sham cultures were established from the same biological samples as stretch-grown cultures, which were used to calculate fold-changes relative to stretch-grown cultures.
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| Contributor(s) |
Loverde JR, Soteropoulos P, Pfister BJ |
| Citation(s) |
33408609 |
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| Submission date |
May 04, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Joseph R. Loverde |
| E-mail(s) |
axonstretch@me.com
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| Organization name |
New Jersey Institute of Technology
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| Department |
Department of Biomedical Engineering
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| Lab |
Center for Injury Biomechanics, Materials, and Medicine
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| Street address |
111 Lock Street
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| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07103 |
| Country |
USA |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM2598364 |
Ipsilateral cervical DRGs, Stretch-induced axon growth, rep 1 |
| GSM2598365 |
Ipsilateral cervical DRGs, Stretch-induced axon growth, rep 2 |
| GSM2598366 |
Ipsilateral cervical DRGs, Stretch-induced axon growth, rep 3 |
| GSM2598367 |
Ipsilateral cervical DRGs, Stretch-induced axon growth, rep 4 |
| GSM2598368 |
Ipsilateral cervical DRGs, Stretch-induced axon growth, rep 5 |
| GSM2598369 |
Paired contralateral cervical DRGs, Static sham, rep 1 |
| GSM2598370 |
Paired contralateral cervical DRGs, Static sham, rep 2 |
| GSM2598371 |
Paired contralateral cervical DRGs, Static sham, rep 3 |
| GSM2598372 |
Paired contralateral cervical DRGs, Static sham, rep 4 |
| GSM2598373 |
Paired contralateral cervical DRGs, Static sham, rep 5 |
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| Relations |
| BioProject |
PRJNA385385 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE98535.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| GSE98535_RAW.tar |
44.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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