|
| Status |
Public on May 05, 2017 |
| Title |
Genome-wide analysis of keratinocytes laser captured from skin biopsies during the mucocutaneous HSV-2 reactivation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We aim to analyze the transcriptional profiles of keratinocytes near dermal-epidemal junction during HSV-2 reactivation in humans, including lesion, post healed (4 and 8 weeks post healing) and contra-lateral control biopsies. Since keratinocytes are the main target cells for HSV-2 infection, the goal is to define the intrinsic immunity of keratinocytes during HSV-2 reactivation in humans.
|
| |
|
| Overall design |
CD8 cells were immunofluorescently stained and keratinocytes above base membrane were laser captured from genital skin biopsies at lesion, 4 and 8 weeks post healed and contra-lateral controls; total RNA were purified from captured cells and cDNA were amplified from the total RNA using NuGEN pico kits.
|
| |
|
| Contributor(s) |
Peng T |
| Citation(s) |
28663436, 34552595 |
| |
| Submission date |
May 04, 2017 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Tao Peng |
| E-mail(s) |
tpeng@u.washington.edu
|
| Phone |
206-667-5861
|
| Organization name |
University of Washington
|
| Department |
Medicine
|
| Street address |
1100 Fairview Ave N/FHCRC/E5-110
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10904 |
Illumina HumanHT-12 V4.0 expression beadchip (gene symbol) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA385479 |
Less...
More...