 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 01, 2017 |
| Title |
RNA-seq analysis of scaffold attachment factor A (SAF-A) knockdown in RPE1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Analysis of gene expression by RNA-seq upon siRNA mediated knockdown of scaffold attachment factor A (SAF-A) versus control siRNA in RPE1 cells at 24 hour and 48 hour time points post transfection reveals SAF-A loss does not impact on gene transcription
|
| |
|
| Overall design |
Two control RNA-seq libraries where produced (24h and 48h) to compare to the two SAF-A siRNA knockdown RNA-seq libraries, each was a single experimental replicate.
|
| |
|
| Contributor(s) |
Nozawa R, Boteva L, Soares DC, Naughton C, Dun AR, Buckle A, Ramsahoye B, Bruton PC, Saleeb RS, Arnedo M, Hill B, Duncan RR, Maciver SK, Gilbert N |
| Citation(s) |
28622508 |
| |
| Submission date |
May 04, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
nick gilbert |
| E-mail(s) |
nick.gilbert@ed.ac.uk
|
| Phone |
00 44 131 651 8551
|
| Organization name |
University of Edinburgh
|
| Department |
MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine
|
| Street address |
Crewe Rd
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (4)
|
| GSM2598423 |
RPE1 cell siRNA Control 24h RNA-seq library |
| GSM2598425 |
RPE1 cell siRNA SAFA 24h RNA-seq library |
| GSM2598426 |
RPE1cell siRNA Control 48h RNA-seq library |
| GSM2598427 |
RPE1 cell siRNA SAFA 48h RNA-seq library |
|
| Relations |
| BioProject |
PRJNA385478 |
| SRA |
SRP106457 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE98541_RAW.tar |
29.2 Mb |
(http)(custom) |
TAR (of GTF, TXT) |
| GSE98541_RPE1_diff_expression_WT_SAFA.xlsx |
12.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...