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| Status |
Public on Apr 11, 2018 |
| Title |
The DNA methylation landscape of enhancers in the guinea pig hippocampus |
| Organism |
Cavia porcellus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
Aim: To determine the state of methylation of DNA molecules that are physically engages with either poised or active enhancers.
Methods: We used ChIP-bisulfite-sequencing with H3K4me1 antibody to map the methylation of DNA bound to enhancer. We used ChIP-sequencing with RNApolII-PS5 antibody to map actively transcribing transcription start sites. We used reduced representation bisulfite sequencing (RRBS) to map total DNA methylation.
Results - conclusion: DNA molecules that are physically found in H3K4me1 chromatin are hypomethylated while DNA found in enhancers that are associated with active transcription is further demethylated.
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| Overall design |
ChIP bisulfite sequencing (H3K4me1 antibody), ChIP sequencing (RNApolII-PS5 antibody) and and RRBS were performed on hippocampus of 3 different wild type 12-week old guinea pig animals.
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| Contributor(s) |
Boureau L, Constantinof A, Moisiadis VG, Matthews SG, Szyf M |
| Citation(s) |
29616589 |
| |
| Submission date |
May 04, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Lisa Boureau |
| E-mail(s) |
boureau.lisa@gmail.com
|
| Phone |
5143987107
|
| Organization name |
McGill University
|
| Department |
Pharmacology
|
| Lab |
Moshe Szyf
|
| Street address |
Sir William Osler Promenade
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
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| Platforms (1) |
| GPL17296 |
Illumina HiSeq 2000 (Cavia porcellus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA385487 |
| SRA |
SRP106466 |
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