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| Status |
Public on May 11, 2008 |
| Title |
Monitoring the ethanol stress response of a sigM deletion strain of B. cereus ATCC 14579. |
| Organism |
Bacillus cereus ATCC 14579 |
| Experiment type |
Expression profiling by array
|
| Summary |
Here, the role of σM and its regulon in stress response and survival of B. cereus ATCC 14579 was assessed by comparative transciptome and phenotypic analysis of this strain and its sigM deletion strain. Exposure of B. cereus ATCC 14579 to a wide range of stresses revealed expression of sigM, encoding σM, to be up-regulated mainly in the presence of ethanol and after alkaline pH-shock. Next to this, disc diffusion tests showed the sigM deletion strain to be more sensitive to oxidizing agents and to be more resistant to cell-wall targeting antibiotics than the wild-type strain. The σM regulon was subsequently determined by comparative transcriptional analyses of the wild-type and its sigM-deletion strain after exposure to ethanol. The putative σM-regulon was shown to consist of 29 genes, several of these genes are predicted to be involved in counteracting oxidative stress, such as an NADH oxidase, a ferredoxin, and a lysine decarboxylase or could encode enzymes involved in methionine metabolism, leading toward L-cysteine production, including luxS. Screening of promoter upstream regions allowed for the assessment of a B. cereus consensus promoter binding site for σM. Since the consensus promoter binding site for B. cereus ATCC 14579 σM, its regulon and the predicted functionalities are different from the corresponding features in B. subtilis, it can be concluded that σM plays a unique role in B. cereus stress response and survival.
Keywords: Stress response, comparative transcriptome study
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| Overall design |
The sigM deletion strain of B. cereus ATCC 14579 was cultured to an OD600 of ~0.6. Here the first RNA sample was taken, 0 min. After sampling 4% of ethanol was added (v/v), and samples were taken after 10, 30 and 60 minutes of exposure. Comparisons performed were 0 min - 10 min, 0 min - 30 min, and 0 min - 60 min.
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| Contributor(s) |
van der Voort M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 12, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Menno van der Voort |
| Organization name |
Wageningen UR
|
| Department |
Agrotechnology and Food Sciences Group
|
| Lab |
Food Microbiology
|
| Street address |
Bomenweg 2
|
| City |
Wageningen |
| ZIP/Postal code |
6703 HD |
| Country |
Netherlands |
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| Platforms (1) |
| GPL4406 |
Bacillus cereus ATCC14579 design1 |
|
| Samples (6)
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| GSM249065 |
Delta sigM 10 min after exposure to ethanol (dM0met10_1) |
| GSM249067 |
Delta sigM after 30 min of exposure to ethanol (dM0met30_1) |
| GSM249069 |
Delta sigM 60 min after ethanol exposure (dM0met60_1) |
| GSM249070 |
Delta sigM 10 minutes after ethanol exposure (dM0met10_2) |
| GSM249079 |
delta sigM after 30 min of ethanol exposure (dM0met30_2) |
| GSM249080 |
delta sigM 60 min after ethanol (dM0met60_2) |
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| Relations |
| BioProject |
PRJNA103835 |
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