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| Status |
Public on May 11, 2008 |
| Title |
Monitoring the ethanol stress response of a sigZ deletion strain of B. cereus ATCC 14579. |
| Organism |
Bacillus cereus ATCC 14579 |
| Experiment type |
Expression profiling by array
|
| Summary |
The stress response of as sigZ deletion strain of B. cereus ATCC 14579 is monitored true time by use of microarrays. The sigZ regulon in ethanol stress response was determined and compared with the regulon determined by micorarray analysis of overexpression of sigZ.
Keywords: stress response, comparative transcriptome study
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| Overall design |
The sigZ deletion strain of B. cereus ATCC 14579 was cultured to an OD600 of ~0.6. Here the first RNA sample was taken, 0 min. After sampling 4% of ethanol was added (v/v), and samples were taken after 10, 30 and 60 minutes of exposure. Comparisons performed were 0 min - 10 min, 0 min - 30 min, and 0 min - 60 min.
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| Contributor(s) |
van der Voort M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 12, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Menno van der Voort |
| Organization name |
Wageningen UR
|
| Department |
Agrotechnology and Food Sciences Group
|
| Lab |
Food Microbiology
|
| Street address |
Bomenweg 2
|
| City |
Wageningen |
| ZIP/Postal code |
6703 HD |
| Country |
Netherlands |
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| Platforms (1) |
| GPL4406 |
Bacillus cereus ATCC14579 design1 |
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| Samples (6)
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| GSM249082 |
delta sigZ 10 min after ethanol exposure (dZ0met10_1) |
| GSM249085 |
delta sigZ 30 min after ethanol exposure (dZ0met30_1) |
| GSM249087 |
delta sigZ 60 min after ethanol exposure (dZ0met60_1) |
| GSM249090 |
delta sigZ 10 min after ethanol exposure (dZ0met10_2) |
| GSM249092 |
delta sigZ 30 min after ethanol exposure (dZ0met30_2) |
| GSM249093 |
delta sigZ 60 min after ethanol exposure (dZ0met60_2) |
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| Relations |
| BioProject |
PRJNA103839 |
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