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Series GSE98589 Query DataSets for GSE98589
Status Public on Sep 05, 2018
Title Mononuclear phagocytes locally specify and adapt their phenotype in the inflamed central nervous system, postimmunization expression data.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We introduce an in vivo imaging approach that allows us to temporally and spatially resolve the evolution of iNOS and Arginase-positive phagocyte phenotypes in a murine MS model. We show that the polarization of individual phagocytes is established after CNS entry, is dependent on the CNS compartment and can be adapted as inflammatory lesions move from expansion to resolution. Our study thus provides a first real-time analysis of phagocyte specification in the intact CNS.
 
Overall design Cells were isolated from the CNS of Arginase-YFP X iNOS-Tomato-Cre mice at clinical onset of Experimental Autoimmune Encephalomyelitis. Cd11bhigh, CD45high, CD64+ cells were FACS sorted based on their relative expression of the reporter proteins YFP and Tomato, indicative of M2 and M1 macrophage polarization, respectively. Total RNA was extracted from the separated YFP+, Tomato+, YFP+Tomato+ and unpolarized populations.
 
Contributor(s) Locatelli G, Theodorou D, Jordao MJ, Staszewski O, Bessis A, Prinz M, Kerschensteiner M
Citation(s) 30127427
Submission date May 05, 2017
Last update date May 15, 2019
Contact name Ori Staszewski
E-mail(s) ori.staszewski@uniklinik-freiburg.de
Organization name University Medical Center Freiburg
Department Institute of Neuropathology
Street address Breisacher Str. 64
City Freiburg
ZIP/Postal code D-79106
Country Germany
 
Platforms (1)
GPL15103 Illumina HiSeq 1000 (Mus musculus)
Samples (16)
GSM2601471 B1_P1_YFP+ cells
GSM2601472 B1_P2_Tomato+ YFP+ cells
GSM2601473 B1_P3_Unpolarized
This SubSeries is part of SuperSeries:
GSE107792 Mononuclear phagocytes locally specify and adapt their phenotype in the inflamed central nervous system
Relations
BioProject PRJNA385606

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98589_Voom_Matrix.txt.gz 2.2 Mb (ftp)(http) TXT
GSE98589_counts.txt.gz 3.9 Mb (ftp)(http) TXT
GSE98589_cpm.txt.gz 2.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data are available on Series record

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