|
| Status |
Public on Dec 26, 2018 |
| Title |
Impact of DNA demethylation agents (5-azacytidine or vitamin C) on gene expression in glioblastoma HSR-GBM1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Glioblastoma HSR-GBM1 cells have low mitochondrial DNA copy numbers which is associated with the abnormal DNA methylation patterns. By inducing DNA demthylation using 5azacytidine and vitamin C, HSR-GBM1 cells modulate their mitochondrial copy number and capability of differentiation.
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| |
|
| Overall design |
HSR-GBM1 cell line with three conditions: untreated control group, treated with 5 azacitidine and treated with vitamin C. 3 biological replicates of each condition.
|
| |
|
| Contributor(s) |
St. John J, Sun X |
| Citation(s) |
29722878 |
| |
| Submission date |
May 09, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Justin C St. John |
| E-mail(s) |
justin.stjohn@hudson.org.au
|
| Phone |
+61399024749
|
| Organization name |
Hudson Institute of Medical Research
|
| Department |
Centre for Genetic Diseases
|
| Lab |
Mitochondrial Genetics
|
| Street address |
27-31 Wright St
|
| City |
Clayton |
| State/province |
Vic |
| ZIP/Postal code |
3168 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (9)
|
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| This SubSeries is part of SuperSeries: |
| GSE98693 |
Induced DNA demethylation, genome instability and transcription |
|
| Relations |
| BioProject |
PRJNA385927 |
| SRA |
SRP106689 |
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