|
| Status |
Public on Jun 30, 2017 |
| Title |
JQ1 prevents BRD4 recruitment to the lytic origins of replication [Akata-Zta DNA-seq] |
| Organisms |
Homo sapiens; human gammaherpesvirus 4 |
| Experiment type |
Other
|
| Summary |
Lytic infection by the Epstein-Barr virus (EBV) poses numerous health risks, such as infectious mononucleosis and lymphoproliferative disorder. We demonstrate that JQ1 and other BET inhibitors block two different steps in the sequential cascade of the EBV life cycle: expression of the immediate-early gene BZLF1 and lytic genome replication. This represents a novel mode of action for antiviral drugs that may increase efficacy and decrease emergence of resistance. The ChIP-seq data below show that the BET proteins bind to both EBV lytic origins of replication.
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| |
|
| Overall design |
DNA-seq of viral DNA in latent LGNFR- and reactivated LGNFR+ Akata-ZTA cells pre-treated with acyclovir and either JQ1 or vehicle
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| |
|
| Contributor(s) |
Keck KM, Moquin SA, Miranda JL |
| Citation(s) |
28588024 |
| |
| Submission date |
May 10, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
JJ Miranda |
| E-mail(s) |
jmiranda@barnard.edu
|
| Organization name |
Barnard College, Columbia University
|
| Street address |
3009 Broadway
|
| City |
New York |
| ZIP/Postal code |
10027 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23362 |
Illumina HiSeq 4000 (Homo sapiens; Human gammaherpesvirus 4) |
|
| Samples (8)
|
| GSM2611566 |
TotalDNA-seq_Akata-Zta+acy+vehicle_LGNFR+_Replicate1 |
| GSM2611567 |
TotalDNA-seq_Akata-Zta+acy+vehicle_LGNFR-_Replicate1 |
| GSM2611568 |
TotalDNA-seq_Akata-Zta+acy+JQ1_LGNFR+_Replicate1 |
| GSM2611569 |
TotalDNA-seq_Akata-Zta+acy+JQ1_LGNFR-_Replicate1 |
| GSM2611570 |
TotalDNA-seq_Akata-Zta+acy+vehicle_LGNFR+_Replicate2 |
| GSM2611571 |
TotalDNA-seq_Akata-Zta+acy+vehicle_LGNFR-_Replicate2 |
| GSM2611572 |
TotalDNA-seq_Akata-Zta+acy+JQ1_LGNFR+_Replicate2 |
| GSM2611573 |
TotalDNA-seq_Akata-Zta+acy+JQ1_LGNFR-_Replicate2 |
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| This SubSeries is part of SuperSeries: |
| GSE84214 |
BET inhibitors block the Epstein-Barr virus lytic cycle at two distinct steps |
|
| Relations |
| BioProject |
PRJNA386166 |
| SRA |
SRP106790 |
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