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Status |
Public on Jul 10, 2018 |
Title |
Open chromatin in somatic cells transplanted into Xenopus oocytes |
Organisms |
Mus musculus; Mus musculus musculus x Mus musculus castaneus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Active gene transcription is associated with an open chromatin configuration at gene regulatory regions. However, the relationship between chromatin architecture of somatic cells and transcriptional activation during nuclear reprogramming in oocytes is unclear. We therefore examined changes in open chromatin states before and after transplantation of mouse somatic nuclei into Xenopus laevis oocytes by using the Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq).
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Overall design |
ATAC-seq was performed using the following cell samples; (1) C2C12 cells, (2) mouse embryonic fibroblasts (MEFs) before and after nuclear transplantation into Xenopus laevis oocytes.
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Contributor(s) |
Miyamoto K, Bradshaw C |
Citation(s) |
29996092 |
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Submission date |
May 10, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Charles Bradshaw |
Organization name |
University of Cambridge
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Department |
Gurdon Institute
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Street address |
Tennis Court Road
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City |
Cambridge |
ZIP/Postal code |
CB2 1QN |
Country |
United Kingdom |
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Platforms (2) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
GPL22270 |
Illumina HiSeq 2500 (Mus musculus musculus x M. m. castaneus) |
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Samples (8)
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Relations |
BioProject |
PRJNA386179 |
SRA |
SRP106806 |