|
| Status |
Public on Feb 25, 2018 |
| Title |
Reduction of rRNA during sRNA library construction using a set of blocking oligos, in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
This experiment was designed to improve the proportion of miRNA mapping reads from sRNA library sequencing, by reducing the amount of particularly abundant rRNA fragments.
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| Overall design |
We extracted the sRNA fraction from 50 pooled male D. melanogaster accesory glands and constructed 4 cDNA libraries from the resultant RNA (blocked vs not-blocked with 2 biogical replicates). For the blocked libraries we introduced several blocking oligos, with sequences complentary to the most abundant contaminating rRNA fragments, with the aim of preventing ligation of that rRNA to library adapters. The other libraries (unblocked) were constructed according to standard HD protocol. The libraries were sequenced on a HighSeq2500 platform.
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| |
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| Contributor(s) |
Fowler E, Mohorianu I, Chapman T |
| Citation(s) |
29474379 |
| |
| Submission date |
May 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
irina.mohorianu@paediatrics.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Medical Sciences Division
|
| Lab |
Oxford Vaccine Group
|
| Street address |
Headington
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7LE |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE98833 |
Reduction of rRNA during sRNA library construction using blocking oligos |
|
| Relations |
| BioProject |
PRJNA386333 |
| SRA |
SRP106904 |
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